analysis of glucose levels in blood

analysis of glucose levels from blood samples oksalated, that blood has been mixed with oxalic acid as an anti-coagulant. Other anti-coagulants used as an anti yangd apat koagualan is EDTA or heparin. In the determination of blood glucose levels, a protein that is also contained in the blood must be precipitated or denatured first. After the process of blood before undergoing a process of heating and the addition of reagents and reagent Cu alkanis fosfomolibdat to give a blue color varies depending on the concentration of the molecules. color variation is then measured with a spectrophotometer absorbannya formed to determine the concentration. determination is quite simple, namely by using a calibration curve of absorbance of Cu alkanis measurable.

In addition BaOH2 little clot of blood samples. BaOH2 used must be in a fresh state before the solution reacts with the surrounding air and form a white layer. BaOH2 addition of this function is as an alkaline conditions and also precipitate the iron ions in the blood. One of the precipitated iron ions are iron ions in hemoglobin. Iron ions are converted into molecules of Fe (OH) 2 form a red precipitate. In the administration of ZnSO4 the red sediment samples terkoagulasi become more and more and more pale. The addition of ZnSO4 serves to precipitate proteins and also mendenaturasi perfectly. Because the weight of the proteins that leih of glucose specific gravity that will subsequently be analyzed then separation by centrifugation. In this process the supernatant formed a more translucent red and brownish precipitate proteins. Deposition is intended for memisakhan protein from glucose because it would interfere with measurements.

Determination of levels is then performed by spectrophotometry. To measure the absorbance then added Cu alkanis molybdate and arsenic. Absorbance is proportional to the glucose concentration to be measured. The more intense blue color of the solution, the greater the concentration of glucose contained.

Glucose levels that are known this can help us predict metabolismeme that may occur in cells with the sugar content is available. L If the content of a large excess of glucose in the body will experience the glucose catabolism of the enzymatic reaction to produce energy. However, if the glucose content is below the minimum threshold, then the pyruvic acid resulting from the process may undergo a process of enzymatic catabolism in anabolism via gluconeogenesis to synthesize glucose and normal glucose levels meet in the blood (serum or blood plasma salam) is 65-110 mg / dL ( 3.6 to 6.1 mmol / L),